The latest medical research on Microbiology

The research magnet gathers the latest research from around the web, based on your specialty area. Below you will find a sample of some of the most recent articles from reputable medical journals about microbiology gathered by our medical AI research bot.

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Simultaneous detection of JAK2, CALR, and MPL mutations and quantitation of JAK2 V617F allele burden in myeloproliferative neoplasms using the quenching probe-Tm method in i-densy IS-5320.

Biochemistry

Accurate detection of myeloproliferative neoplasms (MPN)-associated gene mutations is necessary to correctly diagnose MPN. However, conventional gene testing has various limitations, including the requirement of skilled technicians, cumbersome experimental procedures, and turnaround time of several days. The gene analyzer i-densy IS-5320 allows gene testing using the quenching probe-Tm method. Specifically, pretreatment of samples including DNA extraction, amplification and detection of genes, and analysis of results are performed in a fully automatic manner after samples and test reagents are added into this system, which is compact and can be easily installed in a laboratory. The aim of this study is to investigate the sensitivity and specificity associated with the simultaneous detection of MPN-associated gene mutations.

We conducted an analysis of MPN-associated genes using i-densy IS-5320. We analyzed 384 samples (171 JAK2 V617F mutations, 10 JAK2 exon12 mutations, 104 CALR mutations, and 26 MPL mutations) that had been examined using conventional approaches such as allele-specific polymerase chain reaction (PCR), droplet digital PCR, and the direct sequencing method.

The detection accuracy of JAK2 V617F, JAK2 exon 12, CALR, and MPL was 100.0% (383/383), 99.7% (383/384), 100.0% (370/370), and 99.7% (377/378), respectively. There was a strong positive correlation between the JAK2 V617F allele burden measured using conventional methods and i-densy IS-5320 (r = .989).

Overall, i-densy IS-5320 exhibited good accuracy in terms of analyzing MPN-associated genes; thus, it can serve as a replacement for conventional methods of MPN-associated gene testing.

RNA m6A Modification in Liver Biology and Its Implication in Hepatic Diseases and Carcinogenesis.

Cellular Physiology

N6-methyladenosine (m6A) is the most prevalent internal modification in eukaryotic RNAs. This modification is regulated by three different factors ...

Vascular Contributions to Cognitive Impairment/Dementia (VCID) in Diabetes: Role of Endothelial Cells and Pericytes.

Cellular Physiology

Vascular contributions to cognitive impairment/dementia (VCID) are a leading cause of dementia, a known neurodegenerative disorder characterized by...

Decorin evokes reversible mitochondrial depolarization in carcinoma and vascular endothelial cells.

Cellular Physiology

Decorin, a small leucine-rich proteoglycan with multiple biological functions, is known to evoke autophagy and mitophagy in both endothelial and ca...

How RNA editing keeps an I on physiology.

Cellular Physiology

Adenosine deaminases acting on RNAs convert adenosines (A) to inosines (I) in structured or double- stranded RNAs. In mammals, this process is wide...

The multifunctional nature of CD103 (αEβ7 integrin) signaling in tissue-resident lymphocytes.

Cellular Physiology

Intestinal tissue-resident lymphocytes are critical for maintenance of the mucosal barrier and to prevent enteric infections. The activation of the...

Emerging roles of HMGB1-related lncRNA: from molecular biology to clinical application.

Cellular Physiology

HMGB1 not only induces cell proliferation and migration, but also promotes cell apoptosis and autophagy. Abnormal expression of HMGB1 in plasma or ...

The family of kallikrein-related peptidases and kinin peptides as modulators of epidermal homeostasis.

Cellular Physiology

The epidermis is the outermost skin layer and is part of one of the largest organs in the body; it is supported by the dermis, a network of fibrils...

Procoagulant Platelets: Novel Players in Thromboinflammation.

Cellular Physiology

Platelets play a key role in maintaining hemostasis. However, dysregulated platelet activation can lead to pathological thrombosis or bleeding. Onc...

Alterations in glycosaminoglycan biosynthesis associated with the Ehlers-Danlos Syndromes.

Cellular Physiology

Proteoglycans consist of a core protein substituted with one or more glycosaminoglycan (GAG) chains and execute versatile functions during many phy...

The Role of the Glypican and Syndecan Families of Heparan Sulfate Proteoglycans in Cardiovascular Function and Disease.

Cellular Physiology

Heparan sulfate proteoglycans (HSPGs) are proteoglycans formed by a core protein to which one or multiple heparan sulfate chains are covalently bou...

Impaired regenerative capacity contributes to skeletal muscle dysfunction in chronic obstructive pulmonary disease (COPD).

Cellular Physiology

Locomotor skeletal muscle dysfunction is a relevant comorbidity of chronic obstructive pulmonary disease (COPD) and is strongly associated with wor...